Loss of WDR-60 results in a severe accumulation of KAP-1 near the base of cilia but only has a modest effect on the distribution of OSM-3. Related to Fig. 4. (A) IFT-74::GFP kymographs of a phasmid cilia from control and wdr-60(null) worms. Single channels of particles moving anterogradely and retrogradely are shown, together with their respective merge. (B) Examples of phasmid cilia from control and wdr-60(null) worms expressing KAP-1::GFP and quantification of the signal intensity along cilia (n ≥ 60 cilia for each strain). The intensity of KAP-1::GFP in cilia is significantly increased in the wdr-60(null) mutant with particles accumulating near the TZ. (C) Examples of phasmid cilia from control and wdr-60(null) worms expressing OSM-3::mCherry and quantification of the signal intensity along cilia (n ≥ 88 cilia for each strain). OSM-3::mCherry distribution and intensity along the cilium is only slightly altered in the wdr-60(null) mutant. XY intensity distribution graphs are shown as mean ± SEM. Scale bars: vertical 5 s, horizontal 2 µm (A); 2 µm (B and C).