WDR-60–associated phenotypes become worse as the animals develop to adulthood and do not improve with aging. Related to Fig. 3. (A, C, E, and G) GFP::CHE-3-expressing phasmid cilia of wild-type and wdr-60(null) mutants at several stages of development and aging. L2, larval stage 2 worms; YA:,young adult worms; 7d, worms 7 days after reaching adulthood; 18d, worms 18 days after reaching adulthood. Scale bars: 2 µm. (B, D, F, and H) Corresponding distribution of GFP::CHE-3 signal intensity along cilia. Gray rectangles highlight the TZ, as previously defined. n = 77 cilia for B, n ≥ 95 cilia for D, n ≥ 113 cilia for F, and n ≥ 110 cilia for H. XY intensity distribution graphs are shown as mean ± SEM. (I) Column graph showing GFP::CHE-3 total intensity from the base to the tip of cilia from wild-type and wdr-60(null) mutants analyzed in B, C, F, and H. Graph is shown as mean ± SD. Mann–Whitney U test was used to analyze these datasets. **, P ≤ 0.01; ****, P ≤ 0.0001.