WDR-60::3xFLAG::GFP expression is restricted to ciliated sensory neurons. Related to Figs. 2 and 3. (A and B) Endogenously tagged WDR-60::3xFLAG::GFP and WDR-60(ΔCT)::3xFLAG::GFP are expressed in the same amphid (A) and phasmid (B) ciliated neurons that incorporate the DiI lipophilic dye (in red). The wdr-60(null)::3xflag::gfp strain has no detectable GFP signal in its neurons; however, they are still able to take up the DiI dye. Scale bars: 10 µm. DIC, differential interference contrast. (C) GFP::CHE-3 intensity in soma of phasmid sensory neurons of untagged wdr-60 mutants (n ≥ 45 somas). Graph is shown as mean ± SD. Kruskal–Wallis test followed by the Dunn’s multiple comparison were used to analyze these datasets. *, P ≤ 0.05.