Figure 4.
VAMP4 deficiency leads to the accumulation of other granule cargoes. (A–D) Representative Western blot images showing the expression levels of PC1 (A) and PC2 (C) in WT, KD, KO, and rescued INS-1 cells. The bar graphs show the expression levels of PC1 (B) and PC2 (D) quantified from three independent Western blot results. Actin was used as a loading control. (E and F) RT–PCR analysis of the Pc1 (E) and Pc2 (F) mRNA levels in WT, KD, KO, and rescued INS-1 cells. 18S ribosomal RNA was used as an internal control (n = 3 biological independent experiments). (G) Representative Western blot images showing the expression levels of VAMP2, VAMP7, and VAMP8 in WT, KD, KO, and rescued INS-1 cells. Actin was used as the loading control. The data are presented as the mean ± SEM. *, P < 0.05; **, P < 0.01; and ***, P < 0.001. The statistical analyses were performed with one-way ANOVA (B and D–F). Source data are available for this figure: SourceData F4.

VAMP4 deficiency leads to the accumulation of other granule cargoes. (A–D) Representative Western blot images showing the expression levels of PC1 (A) and PC2 (C) in WT, KD, KO, and rescued INS-1 cells. The bar graphs show the expression levels of PC1 (B) and PC2 (D) quantified from three independent Western blot results. Actin was used as a loading control. (E and F) RT–PCR analysis of the Pc1 (E) and Pc2 (F) mRNA levels in WT, KD, KO, and rescued INS-1 cells. 18S ribosomal RNA was used as an internal control (n = 3 biological independent experiments). (G) Representative Western blot images showing the expression levels of VAMP2, VAMP7, and VAMP8 in WT, KD, KO, and rescued INS-1 cells. Actin was used as the loading control. The data are presented as the mean ± SEM. *, P < 0.05; **, P < 0.01; and ***, P < 0.001. The statistical analyses were performed with one-way ANOVA (B and D–F). Source data are available for this figure: SourceData F4.

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