N-Rok::2m22 is a functional enzyme and efficiently phosphorylates Sqh::mCherry in vivo in a tissue-specific manner. Panels show lateral views of fixed sqh_Sqh::GFP (top panel) or sqh_Sqh::mCherry (bottom panel) embryos at stage 13–14 (dorsal closure) expressing N-Rok::2m22 in the en domain (visualized by co-expression of mCherry-nls in the top panel). Embryos were stained with anti-phospho-Sqh/MRLC antibody. The panel on the right shows magnification of the respective Sqh and p-Sqh images for the presented embryos. Yellow arrows point to Sqh::mCherry and p-Sqh foci. The number of embryos analyzed is indicated (n). Scale bars, 20 μm; in the zoomed panel, 10 μm.