All panels show stills from live-imaging with dorsal views of the developing sqh_Sqh::GFP or sqh_Sqh::mCherry embryos at stages 13/14–16 (dorsal closure) expressing variants of the synthetic kinase or the vhhGFP4 binder alone in the en domain (visualized by co-expression of mCherry-nls). Bottom panel shows dorsal views of embryo expressing SqhE20E21 under the control of enGal4 driver. Yellow arrows point to the Sqh::mCherry foci and actomyosin cable invaginations in N-Rok::2m22 panel. Please note that the “control” image is the same as on Figs. 3 a, 4 b, and Fig. S1, to have a single reference image for all N-Rok variants used. For every genotype, the number of considered embryos is indicated (n). Scale bars, 50 μm.