Figure S1.
Binding of vhhGFP4 to GFP results in an increase of the fluorescence signal. All panels show stills from live-imaging with dorsal views of either sqh_Sqh::GFP or sqh_Sqh::mCherry embryos at stage 13/14 (dorsal closure) expressing either N-RokDead variants or vhhGFP4 nanobody alone in the en domain (visualized by co-expression of mCherry-nls). Note the enhancement of fluorescent signal due to the binding of vhhGFP4 or 2m22 to GFP and to mCherry, respectively. The right panel shows magnification of the respective images for each of the embryo genotypes shown on the left. Please note that the “control” image is the same as on Figs. 3 a and 4 b to have a single reference image for all N-Rok variants used. For every expressed synthetic kinase, the number of considered embryos is indicated (n). Scale bars, 50 μm; in the zoomed panel, 10 μm.

Binding of vhhGFP4 to GFP results in an increase of the fluorescence signal. All panels show stills from live-imaging with dorsal views of either sqh_Sqh::GFP or sqh_Sqh::mCherry embryos at stage 13/14 (dorsal closure) expressing either N-RokDead variants or vhhGFP4 nanobody alone in the en domain (visualized by co-expression of mCherry-nls). Note the enhancement of fluorescent signal due to the binding of vhhGFP4 or 2m22 to GFP and to mCherry, respectively. The right panel shows magnification of the respective images for each of the embryo genotypes shown on the left. Please note that the “control” image is the same as on Figs. 3 a and 4 b to have a single reference image for all N-Rok variants used. For every expressed synthetic kinase, the number of considered embryos is indicated (n). Scale bars, 50 μm; in the zoomed panel, 10 μm.

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