Figure S2.
Characterization of the CLiP strain (LMJ.RY0420.182282) named arl3-282. (A) Agarose gel electrophoresis of the PCR products amplified from genomes of CC-5325 and arl3-282 cells. The primer pair gARL3-FOR and gARL3-REV were used to amplify the ARL3 genomic DNA of 624-bp from CC-5325 cells. A single DNA fragment of ∼2.8-kb (2,848-bp) was amplified from the arl3-282 cells by using the same primer pair. (B) Schematic representation showing that a paromomycin-resistant gene (aphVIII) inserted to the fourth exon in ARL3 gene of arl3-282 cells. The boxes with colors and the lines represent the exons and introns of ARL3 gene, respectively. (C) Sequence alignment of the ARL3 genomic DNAs (gDNA) between CC-5325 and arl3-282 cells. The arl3-282 cell contains a 2,284-bp aphVIII insertion between 520- and 521-bp in the ARL3 gDNA. Source data are available for this figure: SourceData FS2.

Characterization of the CLiP strain (LMJ.RY0420.182282) named arl3-282. (A) Agarose gel electrophoresis of the PCR products amplified from genomes of CC-5325 and arl3-282 cells. The primer pair gARL3-FOR and gARL3-REV were used to amplify the ARL3 genomic DNA of 624-bp from CC-5325 cells. A single DNA fragment of ∼2.8-kb (2,848-bp) was amplified from the arl3-282 cells by using the same primer pair. (B) Schematic representation showing that a paromomycin-resistant gene (aphVIII) inserted to the fourth exon in ARL3 gene of arl3-282 cells. The boxes with colors and the lines represent the exons and introns of ARL3 gene, respectively. (C) Sequence alignment of the ARL3 genomic DNAs (gDNA) between CC-5325 and arl3-282 cells. The arl3-282 cell contains a 2,284-bp aphVIII insertion between 520- and 521-bp in the ARL3 gDNA. Source data are available for this figure: SourceData FS2.

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