Figure 3.
The tensin3 IDR is required for the interaction with talin and vinculin. (A) Schematic of the tensin3 deletion mutants used, with indicated truncation sites. (B and C) Tensin3 truncations were expressed as N-terminally tagged GFP fusion constructs together with mCh-vinFL-cBAK or mCh-talinFL-cBAK in NIH3T3 cells. Only those constructs containing the intrinsically disordered region (IDR) of tensin3 linking the PTEN and SH2 domains co-localized with vinculin or talin at mitochondria. (D) A tensin3 IDR-only construct (GFP-tensin3-IDR) co-localizes at mitochondria when co-expressed in NIH3T3 cells with either mCh-vinFL-cBAK or mCh-talinFL-cBAK. (E) GFP-tagged tensin3 truncations expressed in NIH3T3 cells; strongest localization to cell-matrix adhesions is observed in those constructs containing the tensin3 IDR (GFP-tensin3-∆Nterm, GFP-tensin3-∆Cterm, GFP-tensin3-IDR). Scale bars in B, D, and E indicate 10 µm.

The tensin3 IDR is required for the interaction with talin and vinculin. (A) Schematic of the tensin3 deletion mutants used, with indicated truncation sites. (B and C) Tensin3 truncations were expressed as N-terminally tagged GFP fusion constructs together with mCh-vinFL-cBAK or mCh-talinFL-cBAK in NIH3T3 cells. Only those constructs containing the intrinsically disordered region (IDR) of tensin3 linking the PTEN and SH2 domains co-localized with vinculin or talin at mitochondria. (D) A tensin3 IDR-only construct (GFP-tensin3-IDR) co-localizes at mitochondria when co-expressed in NIH3T3 cells with either mCh-vinFL-cBAK or mCh-talinFL-cBAK. (E) GFP-tagged tensin3 truncations expressed in NIH3T3 cells; strongest localization to cell-matrix adhesions is observed in those constructs containing the tensin3 IDR (GFP-tensin3-∆Nterm, GFP-tensin3-∆Cterm, GFP-tensin3-IDR). Scale bars in B, D, and E indicate 10 µm.

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