Tensin3 interaction partners revealed by proximity biotinylation (A and B) and the mitochondrial targeting system (C and D). (A) Scatter plot showing the correlation of the SILAC colocalization log₂ ratio of the two tensin3 BioID repeats (data set in file Table S1). The majority of proteins are background proteins (bottom left of the graph); those on the top right of the graph (SILAC ratio ≥2.5) were considered as enriched. The two repeats were highly reproducible with a correlation coefficient of r = 0.9817. (B) Table listing the proteins identified from the BioID experiments using a cut-off ≥2.5 (Ratio of Heavy/Light). (C) Co-expression of mCh-tensin1-, tensin2-, or tensin3-cBAK with GFP-vinculin in NIH3T3 cells shows that GFP-vinculin co-localizes at mitochondria with both tensin1- and tensin3-cBAK but not tensin2-cBAK. (D) Summary table of which cell-matrix adhesion proteins co-localised with mCh-tensin1-, tensin2-, or tensin3-cBAK in NIH3T3 cells; scale bar indicates 10 µm.