Figure 6. Imaging of Dhc KD cells in the... | Rockefeller University Press

Figure 6.

$Imaging of Dhc KD cells in the follicular epithelium of Drosophila ovaries. In all panels, red asterisks mark KD cells and green asterisks mark WT cells. (A) Overview of the epithelium. Black arrows highlight the reduced space between the oocyte and the KD cell, compared with the neighboring WT epithelium. White arrowheads point to sites of lateral deposition of vitellin membrane-like electron-scattering material. (B and C) Higher magnification of material deposited on the lateral side between two KD cells. The material forms an electron-scattering drop (arrowheads) that does not mix with the surrounding extracellular space, which appears darker. (D) Potential exocytosis event of electron-scattering material on the lateral side of a KD cell (arrowhead). (E) Manual segmentation of the event in D. Plasma membrane of the KD cell is shown in red and the drop of material secreted in white. (F and G) High-magnification details of the microvilli between the oocyte and FCs, as indicated in A by black arrows at lower magnification. VM, vitelline material between FCs and oocyte. Arrowheads indicate the microvilli. (H–P) Characterization of MVBs. A WT cell accumulates large MVBs on its apical side (H, white arrowheads), whereas in KD cells the MVBs are mostly localized toward the basal side (I, black arrowheads). J shows a typical example of an apical MVB in WT FC. K and L show basolateral MVBs in KD cells. M and N show segmentation of MVBs in a WT (green) and a KD (red) cell, respectively. The funnel-like shape of the KD cells is also particularly evident in this example. In O, a box plot of the distribution of the MVBs in WT (n = 5) and KD (n = 5) cells. Each point in the plot represents the fraction of apical MVBs in a cell. The plot shows the median and the first and third quartiles of the distribution. Independent t test was used to compare means. ***, P = 2E−6. (P) The plot shows the size distribution of MVBs in WT and KD FCs. 458 MVBs from five WT cells and 616 MVBs from five KD cells were pooled. Two-sample Kolmogorov-Smirnov test confirmed that the two distributions are different (*, P = 3.06E-4).$

Imaging of Dhc KD cells in the follicular epithelium of Drosophila ovaries. In all panels, red asterisks mark KD cells and green asterisks mark WT cells. (A) Overview of the epithelium. Black arrows highlight the reduced space between the oocyte and the KD cell, compared with the neighboring WT epithelium. White arrowheads point to sites of lateral deposition of vitellin membrane-like electron-scattering material. (B and C) Higher magnification of material deposited on the lateral side between two KD cells. The material forms an electron-scattering drop (arrowheads) that does not mix with the surrounding extracellular space, which appears darker. (D) Potential exocytosis event of electron-scattering material on the lateral side of a KD cell (arrowhead). (E) Manual segmentation of the event in D. Plasma membrane of the KD cell is shown in red and the drop of material secreted in white. (F and G) High-magnification details of the microvilli between the oocyte and FCs, as indicated in A by black arrows at lower magnification. VM, vitelline material between FCs and oocyte. Arrowheads indicate the microvilli. (H–P) Characterization of MVBs. A WT cell accumulates large MVBs on its apical side (H, white arrowheads), whereas in KD cells the MVBs are mostly localized toward the basal side (I, black arrowheads). J shows a typical example of an apical MVB in WT FC. K and L show basolateral MVBs in KD cells. M and N show segmentation of MVBs in a WT (green) and a KD (red) cell, respectively. The funnel-like shape of the KD cells is also particularly evident in this example. In O, a box plot of the distribution of the MVBs in WT (n = 5) and KD (n = 5) cells. Each point in the plot represents the fraction of apical MVBs in a cell. The plot shows the median and the first and third quartiles of the distribution. Independent t test was used to compare means. ***, P = 2E−6. (P) The plot shows the size distribution of MVBs in WT and KD FCs. 458 MVBs from five WT cells and 616 MVBs from five KD cells were pooled. Two-sample Kolmogorov-Smirnov test confirmed that the two distributions are different (*, P = 3.06E-4).

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