Figure 7. Contribution of lateral... | Rockefeller University Press

Figure 7.

$Contribution of lateral diffusion/recycling to protein localization in distal dendrites and spines following global and somatic ER release. (A) Experimental schematic. Cargoes are released from the ER in the absence of cross-linking antibody and allowed to traffic for 90 min before adding Alexa647-anti-GFP to label surface protein. (B) Distribution of surface DHFR-GFP-NL1 and DHFR-GFP-GluA1 following global ER release under cross-linking (anti-GFP present throughout the experiment) and non–cross-linking (anti-GFP added 90 min post-ER release) conditions. The ratio of total surface signal in dendrites (>40 µm from the soma) versus soma is shown in the middle panel. The fraction of spines with each cargo is plotted to the right. n values correspond to the number of cells, from at least two independent experiments (**, P < 0.01; ****, P < 0.0001; Student's t test). (C) Same experiment as in B, except cargoes were locally released from the somatic ER. n values correspond to the number of cells, from at least two independent experiments (*, P < 0.05; ***, P < 0.001; Student's t test).$

Contribution of lateral diffusion/recycling to protein localization in distal dendrites and spines following global and somatic ER release. (A) Experimental schematic. Cargoes are released from the ER in the absence of cross-linking antibody and allowed to traffic for 90 min before adding Alexa647-anti-GFP to label surface protein. (B) Distribution of surface DHFR-GFP-NL1 and DHFR-GFP-GluA1 following global ER release under cross-linking (anti-GFP present throughout the experiment) and non–cross-linking (anti-GFP added 90 min post-ER release) conditions. The ratio of total surface signal in dendrites (>40 µm from the soma) versus soma is shown in the middle panel. The fraction of spines with each cargo is plotted to the right. n values correspond to the number of cells, from at least two independent experiments (**, P < 0.01; ****, P < 0.0001; Student's t test). (C) Same experiment as in B, except cargoes were locally released from the somatic ER. n values correspond to the number of cells, from at least two independent experiments (*, P < 0.05; ***, P < 0.001; Student's t test).