Blockade of INAVA and FUS, FMR1, and FXR1 condensates. (A) HeLa cells stably expressing FUS-GFP were pretreated with OTSSP167 (5 μM), INK128 (10 μM), or SB203580 (10 μM) for 30 min followed by sodium arsenate (1 mM) for 90 min. Images were acquired at six positions/well at 20× magnification, mean ± SD, n = 6. Reproduced in three independent studies. (B and C) As in A but with WT HeLa cells treated with MG132 (10 μM) for 5 h. Cells were fixed and stained with (B) α-FMR1 or (C) α-FXR1. (D) FUS-GFP images from A. (E) Images of FMR1 and FXR1 from B and C. Scale bars = 10 μm. *, P < 0.05; **, P < 0.01; ****, P < 0.0001.