Figure 5. Inhibitors of the MAPK p38α... | Rockefeller University Press

Figure 5.

Inhibitors of the MAPK p38α and mTOR pathway induce the resolution of INAVA condensates. (A and B) In HCT8-INAVA-GFP cells, IL-1β– and H2O2-induced INAVA-GFP condensates are blocked by mTOR inhibitors (A) and p38α inhibitors (B). Graphs display results from high-content imaging, mean ± SD, n = 2. (C and D) Dose–response curves for (C) mTOR inhibitor INK128 or (D) p38α inhibitor SB203580, mean ± SEM, n = 3. (E) High-content images from dose–response studies. HCT8-INAVA-GFP cells were pretreated with INK128 (43 μM) or SB203580 (14 μM) for 30 min followed by IL-1β (10 ng/ml) for 90 min. Scale bar = 5 μm. (F) Cells were treated with IL-1β (10 ng/ml) for 50 min followed by INK128 (10 μM) or SB203580 (10 μM) then fixed and stained with Hoechst (nuclei) at indicated time points, n = 2 independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.

Inhibitors of the MAPK p38α and mTOR pathway induce the resolution of INAVA condensates. (A and B) In HCT8-INAVA-GFP cells, IL-1β– and H₂O₂-induced INAVA-GFP condensates are blocked by mTOR inhibitors (A) and p38α inhibitors (B). Graphs display results from high-content imaging, mean ± SD, n = 2. (C and D) Dose–response curves for (C) mTOR inhibitor INK128 or (D) p38α inhibitor SB203580, mean ± SEM, n = 3. (E) High-content images from dose–response studies. HCT8-INAVA-GFP cells were pretreated with INK128 (43 μM) or SB203580 (14 μM) for 30 min followed by IL-1β (10 ng/ml) for 90 min. Scale bar = 5 μm. (F) Cells were treated with IL-1β (10 ng/ml) for 50 min followed by INK128 (10 μM) or SB203580 (10 μM) then fixed and stained with Hoechst (nuclei) at indicated time points, n = 2 independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.