ROS and HSP90 and proteasome inhibitors induce INAVA puncta. (A and B) Dose response for HSP90 inhibitors (A) ganetespib and (B) 17AAG, mean ± SEM, n = 3. (C) Time course of puncta formation by IL-1β (10 ng/ml), ganetespib (1 μM) and 17AAG (10 μM), mean ± SD, n = 2. (D) Time course of puncta formation and resolution after treatment with agonists noted above, n = 2. (E) Time course images from D. Scale bar = 10 μm. (F and G) Confocal images of HCT8-INAVA-GFP cells stained for (F) vimentin or (G) G3BP1 following treatment with IL-1β or H₂O₂ for 90 min. Hoechst (nuclei). Line scans (arrows) display relative fluorescence intensity of INAVA and (F) vimentin or (G) G3BP1. Scale bar = 20 μm.