IL-1β–induced INAVA puncta are biomolecular condensates. (A) Time course of condensate formation in HCT8-INAVA-GFP cells treated with IL-1β. Scale bar = 5 μm. (B) Quantification of puncta per 1,000 cells, mean ± SD, n = 2. Images acquired at four positions/well. (C) As in B but analyzed by puncta area. (D) HCT8 cells expressing the long or short isoform of INAVA treated with IL-1β for 90 min. Scale bar = 10 μm. (E) IL-1β–induced puncta fusion over time in HCT8 cells. Scale bar = 2 μm. (F) FRAP of IL-1β–induced puncta at 30 min. Scale bar = 2 μm. (G) FRAP of “young” (30 min) and “old” (90 min) puncta, mean ± SEM, n = 10. (H) Velocity tracking of puncta, mean ± SEM, n = 36. ****, P < 0.0001.