![Differential regulation of KLP-7 dynamics in the anterior and posterior processes of the PLM. (A–D) The ROI schematics (A), kymographs (B), and analysis (C and D) of GFP::KLP-7 dynamics (Video 3) in control and colchicine-treated worms. The green traces of the GFP::KLP-7 movements are illustrated next to the kymographs. N = 4–8 independent replicates. For C, n (number of neurons) = 20–27; for D, n (number of GFP::KLP-7 tracks) = 132–618. Ant, anterior; Pst, posterior. (E–K) Kymographs and analysis of GFP::KLP-7 dynamics in unc-73(0; E, G, and H), KLP-7S546E S555E[+] (F, G, and H) and let-502 RNAi (I, J, and K) backgrounds. N = 4–6 independent replicates. For G and J, n (number of neurons) = 15–27. For H and K, n (number of GFP::KLP-7 tracks) = 383–675. (L) Pathway showing the regulation of KLP-7 dynamics by PAK-1 and ROCK. For C, D, G, H, J, and K, ***, P < 0.001; ANOVA with Tukey’s multiple comparison test. Error bars represent SEM.](https://cdn.rupress.org/rup/content_public/journal/jcb/220/9/10.1083_jcb.202005080/4/jcb_202005080_fig9.png?Expires=1771172052&Signature=SCpC3buLLG2wFtwRHPpsQuFKLpkGamzZBzG9SYt2r3Fbzj1vqjJKvL0PQieozMByx6ashAThFVS~JzsdsNwsdTFmkzenYbySLk9NIGf3gzSnqdBxv-RI5oRjZG0w3r5jbfhWpRIIajllnbQckGbKyG3ZziFf1OCD1Me7awTm7bThaCuU~L3GwHVSjOOlRdWux6Nitu8bKp9lLKsbbhUeGcvCe-6HwgNTfALcgxWgkt1Hr3kZyj~5CxoFFTNGj4YUqcwol~6zMoTkn0lYQZdcTUZteip4qzn3GBGxVpDukU~ezOY-ktak7czfW6PHUL7attM5fvy5wx7yOPuhHJEZow__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Differential regulation of KLP-7 dynamics in the anterior and posterior processes of the PLM. (A–D) The ROI schematics (A), kymographs (B), and analysis (C and D) of GFP::KLP-7 dynamics (Video 3) in control and colchicine-treated worms. The green traces of the GFP::KLP-7 movements are illustrated next to the kymographs. N = 4–8 independent replicates. For C, n (number of neurons) = 20–27; for D, n (number of GFP::KLP-7 tracks) = 132–618. Ant, anterior; Pst, posterior. (E–K) Kymographs and analysis of GFP::KLP-7 dynamics in unc-73(0; E, G, and H), KLP-7S546E S555E[+] (F, G, and H) and let-502 RNAi (I, J, and K) backgrounds. N = 4–6 independent replicates. For G and J, n (number of neurons) = 15–27. For H and K, n (number of GFP::KLP-7 tracks) = 383–675. (L) Pathway showing the regulation of KLP-7 dynamics by PAK-1 and ROCK. For C, D, G, H, J, and K, ***, P < 0.001; ANOVA with Tukey’s multiple comparison test. Error bars represent SEM.
