Figure S5. Regulation of microtubule... | Rockefeller University Press

Figure S5.

$Regulation of microtubule polarity through Wnt PCP components, related to Fig. 8. (A and B) Relative frequency distribution of the fraction of microtubules with plus-end-out polarity in anterior (A) and posterior (B) processes due to the RNAi of klp-7, daam-1, and rho-1. Asterisks indicate the mode value for each distribution, and the gray shaded areas cover the population of the microtubules having fraction polarity value 0.8 or above. N = 3–4 independent replicates; n (number of neurons) = 23–29. (C) Change in the percentage of PLM processes with microtubules oriented either in unipolar (lighter gray) or mixed (darker gray) manner due to expression of KLP-7WT and active version (A-type) of KLP-7 (KLP-7T549E) in WT worms. N= 3 independent replicates; n (number of neurons) = 25–31. Ant, anterior; Pst, posterior. (D) Growth length of the EBP-2 tracks due to the expression of either an activated version of KLP-7 (KLP-7T549E) or RHO-1G14V. N = 3 independent replicates; n (number of tracks) = 170–530. (E) The effect on fraction polarity values of microtubules due to the expression of RHO-1G14V or KLP-7T549E in a lin-17(0) background. N = 3 independent replicates; n (number of neurons) = 20–38. (F) The fraction polarity values of microtubules in unc-73(0) and unc-73(0);klp-7(0) backgrounds. N = 3–4 independent replicates; n (number of neurons) = 19–46. (G and H) Growth length (G) and duration (H) of the EBP-2 tracks, respectively, in unc-73(0). N = 3–4 independent replicates; n (number of tracks) = 170–400. (I) The effect on fraction polarity values of microtubules due to the expression of WT (CED-10WT) or activated CED-10 (CED-10G12V). N = 3 independent replicates; n (number of neurons) = 28–33. (J and K) The change in the frequency distribution of the fraction of microtubules with plus-end-out polarity due to the expression of Pmec-4:CED-10G12V in the anterior (J) and posterior (K) processes of PLM in lin-17(0). N = 3 independent replicates; n (number of neurons) = 26–33. Datasets were compared by χ2 (Fisher’s exact) test (C) and ANOVA with Tukey’s multiple comparison test (D–I). Error bars represent SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001.$

Regulation of microtubule polarity through Wnt PCP components, related to Fig. 8. (A and B) Relative frequency distribution of the fraction of microtubules with plus-end-out polarity in anterior (A) and posterior (B) processes due to the RNAi of klp-7, daam-1, and rho-1. Asterisks indicate the mode value for each distribution, and the gray shaded areas cover the population of the microtubules having fraction polarity value 0.8 or above. N = 3–4 independent replicates; n (number of neurons) = 23–29. (C) Change in the percentage of PLM processes with microtubules oriented either in unipolar (lighter gray) or mixed (darker gray) manner due to expression of KLP-7^WT and active version (A-type) of KLP-7 (KLP-7^T549E) in WT worms. N= 3 independent replicates; n (number of neurons) = 25–31. Ant, anterior; Pst, posterior. (D) Growth length of the EBP-2 tracks due to the expression of either an activated version of KLP-7 (KLP-7^T549E) or RHO-1^G14V. N = 3 independent replicates; n (number of tracks) = 170–530. (E) The effect on fraction polarity values of microtubules due to the expression of RHO-1^G14V or KLP-7^T549E in a lin-17(0) background. N = 3 independent replicates; n (number of neurons) = 20–38. (F) The fraction polarity values of microtubules in unc-73(0) and unc-73(0);klp-7(0) backgrounds. N = 3–4 independent replicates; n (number of neurons) = 19–46. (G and H) Growth length (G) and duration (H) of the EBP-2 tracks, respectively, in unc-73(0). N = 3–4 independent replicates; n (number of tracks) = 170–400. (I) The effect on fraction polarity values of microtubules due to the expression of WT (CED-10^WT) or activated CED-10 (CED-10^G12V). N = 3 independent replicates; n (number of neurons) = 28–33. (J and K) The change in the frequency distribution of the fraction of microtubules with plus-end-out polarity due to the expression of Pmec-4:CED-10^G12V in the anterior (J) and posterior (K) processes of PLM in lin-17(0). N = 3 independent replicates; n (number of neurons) = 26–33. Datasets were compared by χ² (Fisher’s exact) test (C) and ANOVA with Tukey’s multiple comparison test (D–I). Error bars represent SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001.