Downstream components of Wnt signaling regulate microtubule polarity. (A and B) Illustration of the ROIs and the kymographs showing the EBP-2 tracks in control and let-502 hairpin loop (hp) RNAi-expressing neurons. (C and D) The quantification of the effect on microtubule arrangement in the PLM processes due to Pmec-4–driven expression of let-502 (hp) RNA, due to RNAi of genes involving PCP (C), due to expression of the A-type active version of KLP-7 Pmec-4-KLP-7^T549E (shrEX303), and due to the activated version of RHO-1 Pmec-4-RHO-1^G14V (shrEx332) in a lin-17(0) background (D). For C and D, N = 3–4 independent replicates; n (number of neurons) = 20–39. Ant, anterior; Pst, posterior. (E) Kymographs of EBP-2::GFP dynamics in unc-73(0) and unc-73(0);klp-7(0) backgrounds. (F) The percentage of PLM processes with microtubules oriented in either a unipolar or mixed manner in unc-73(0) and unc-73(0);klp-7(0) backgrounds. N = 3–5 independent replicates; n (number of neurons) = 19–46. (G) Kymographs of EBP-2::GFP in lin-17(0) background expressing the WT (shrEx344) or activated version of CED-10 (shrEx346) in touch neurons. (H) Quantification of the percentage of PLMs with microtubules oriented in either a unipolar or mixed manner. N = 3 independent replicates; n (number of neurons) = 28–33. For B, E, and G, the green and magenta traces represent microtubules of plus-end-out and minus-end-out polarity, respectively. For C, D, F, and H, *, P < 0.05; ***, P < 0.001; Fisher’s exact test.