Figure S2. The changes in microtubule... | Rockefeller University Press

Figure S2.

$The changes in microtubule organization and axonal transport in the klp-7 mutant, related to Fig. 2. (A–D) Frequency distribution of the fraction polarity values of microtubules with plus-end-out (A and C) or minus-end-out (B and D) polarity in anterior (A and B) and posterior (C and D) processes of the PLM. Asterisks indicate the mode value for each distribution, and the gray shaded areas represent the population of the microtubules having fraction polarity value 0.8 or above. N = 3–8 independent replicates; n (number of neurons) = 28–63. (E) Confocal images of Pmec-4-GFP::PTRN-1 (juEx6455). Arrowheads indicate the minus end–related enrichment of PTRN-1. (F) Quantification of GFP::PTRN-1 punctae per unit length of ROI of the anterior (Ant) and posterior (Pst) processes of the PLM. N = 3–5 independent replicates; n (number of neurons) = 37–49. (G and H) Histograms depicting length (G) and duration (H) of the GFP::RAB-3 movement events as shown in Fig. 2 I. Anter, anterograde; Retr, retrograde. N = 3–8 independent replicates; n (number of GFP::RAB-3 tracks) = 122–452. Error bars represent SEM. Statistical comparisons were done using ANOVA with Tukey’s multiple comparison test (F–H). *, P < 0.05; ***, P < 0.001.$

The changes in microtubule organization and axonal transport in the klp-7 mutant, related to Fig. 2. (A–D) Frequency distribution of the fraction polarity values of microtubules with plus-end-out (A and C) or minus-end-out (B and D) polarity in anterior (A and B) and posterior (C and D) processes of the PLM. Asterisks indicate the mode value for each distribution, and the gray shaded areas represent the population of the microtubules having fraction polarity value 0.8 or above. N = 3–8 independent replicates; n (number of neurons) = 28–63. (E) Confocal images of Pmec-4-GFP::PTRN-1 (juEx6455). Arrowheads indicate the minus end–related enrichment of PTRN-1. (F) Quantification of GFP::PTRN-1 punctae per unit length of ROI of the anterior (Ant) and posterior (Pst) processes of the PLM. N = 3–5 independent replicates; n (number of neurons) = 37–49. (G and H) Histograms depicting length (G) and duration (H) of the GFP::RAB-3 movement events as shown in Fig. 2 I. Anter, anterograde; Retr, retrograde. N = 3–8 independent replicates; n (number of GFP::RAB-3 tracks) = 122–452. Error bars represent SEM. Statistical comparisons were done using ANOVA with Tukey’s multiple comparison test (F–H). *, P < 0.05; ***, P < 0.001.

This Feature Is Available To Subscribers Only