The role of the biosynthetic pathway in phagosome resolution and degradative capacity of macrophages. (A) RAW cells internalized mRFP1–E. coli for 20 min, and, after a 1-h chase, cells were treated with vehicle or cycloheximide (CHX) for 6 h total. Scale bars: 5 µm. (B) Number of PDVs per cell from A shown as mean ± SEM from groups of 15 images, each containing between 5 and 15 cells, from 3 independent experiments. Statistical analysis by one-way ANOVA with Tukey’s post hoc test (**, P < 0.01; ***, P < 0.001).