Figure 8.
Phagosome resolution recovers degradative capacity in macrophages. (A) Macrophages were given no particles or allowed to engulf for 1 h unlabeled E. coli or unlabeled IgG-opsonized beads for the first round of phagocytosis, followed by 1 h or 6 h to elicit maturation/resolution, before the addition of DQ-BSA–opsonized beads for a second round of engulfment. External beads were immunostained (green), and cells were imaged live. Lower panels show the red channel (DQ-BSA) in fire scale. Scale bars: 10 µm. (B) Quantification of DQ-BSA fluorescence intensity of internalized DQ-BSA beads from experiments described in A. The intensity of each internal DQ-BSA bead was corrected by subtracting the mean intensity of external DQ-BSA beads. Data are presented as mean ± SD normalized to the mean of 1 h per vehicle condition and are based on 6 independent experiments with 150–503 cells quantified per condition: *, P < 0.05; ***, P < 0.001.

Phagosome resolution recovers degradative capacity in macrophages. (A) Macrophages were given no particles or allowed to engulf for 1 h unlabeled E. coli or unlabeled IgG-opsonized beads for the first round of phagocytosis, followed by 1 h or 6 h to elicit maturation/resolution, before the addition of DQ-BSA–opsonized beads for a second round of engulfment. External beads were immunostained (green), and cells were imaged live. Lower panels show the red channel (DQ-BSA) in fire scale. Scale bars: 10 µm. (B) Quantification of DQ-BSA fluorescence intensity of internalized DQ-BSA beads from experiments described in A. The intensity of each internal DQ-BSA bead was corrected by subtracting the mean intensity of external DQ-BSA beads. Data are presented as mean ± SD normalized to the mean of 1 h per vehicle condition and are based on 6 independent experiments with 150–503 cells quantified per condition: *, P < 0.05; ***, P < 0.001.

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