Figure 6.
PDVs exhibit lysosomal properties. (A) Macrophages were challenged with ZsGreen–E. coli for 6 h, fixed, and stained for LAMP1 or LAMP2. Arrows indicate colocalization of LAMP1 or LAMP2 to PDVs. Scale bars: 5 µm (main panels), 1 µm (insets). (B) Percentage of PDVs positive for LAMP1/LAMP2. Data are mean ± SD of 60 cells from 3 independent experiments. (C and E) PDVs from ZsGreen–E. coli phagosomes visualized at 6 and 20 h after phagocytosis in cells stained with LysoTracker Red (C) or Magic red (E) for 1 h before live-cell imaging. Scale bars: 10 µm. Dashed lines show cell contours (A, C, and E). (D and F) PDVs were scored for co-occurrence with Lysotracker Red (D) or Magic Red (F) using images as shown in C and E, respectively. Data are mean ± SEM of 3 independent experiments with 25–70 cells quantified per time point of an experiment. Data were statistically analyzed by an unpaired t test.

PDVs exhibit lysosomal properties. (A) Macrophages were challenged with ZsGreen–E. coli for 6 h, fixed, and stained for LAMP1 or LAMP2. Arrows indicate colocalization of LAMP1 or LAMP2 to PDVs. Scale bars: 5 µm (main panels), 1 µm (insets). (B) Percentage of PDVs positive for LAMP1/LAMP2. Data are mean ± SD of 60 cells from 3 independent experiments. (C and E) PDVs from ZsGreen–E. coli phagosomes visualized at 6 and 20 h after phagocytosis in cells stained with LysoTracker Red (C) or Magic red (E) for 1 h before live-cell imaging. Scale bars: 10 µm. Dashed lines show cell contours (A, C, and E). (D and F) PDVs were scored for co-occurrence with Lysotracker Red (D) or Magic Red (F) using images as shown in C and E, respectively. Data are mean ± SEM of 3 independent experiments with 25–70 cells quantified per time point of an experiment. Data were statistically analyzed by an unpaired t test.

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