Figure 7.
SREBF2 and LDLR rescue cholesterol reduction caused by TDP-43 deletion. (A) MO3.13 cells were transfected with control siRNA and siRNA against TDP-43 or SREBF2. Cells were stained with TDP-43 (red), SREBF2 (green), and filipin (gray). Scale bar: 10 µm. (B) Relative filipin fluorescent intensity in cells treated with control siRNA and siRNA against TDP-43 or SREBF2. Quantification was done from three independent experiments with 90 cells quantified per experiment. One-way ANOVA with Tukey’s multiple comparisons tests was used to evaluate statistical significance; *, P < 0.05. (C) Total cholesterol level as measured by a fluorometric-based assay in cells treated with control siRNA and siRNA against TDP-43 or SREBF2. n = 3, one-way ANOVA with Tukey’s multiple comparisons tests was used to evaluate statistical significance; *, P < 0.05; **, P < 0.01. (D) Schematic of cDNA rescue experiment under TDP-43 knockdown conditions. (E) Immunoblotting for SREBF2 and LDLR in the rescue experiments. Blue arrow: full-length SREBF2; red arrow: transfected N-terminal domain of SREBF2; orange arrow: N-terminal domain of SREBF2; purple arrow: GFP-tagged LDLR; black arrow: endogenous LDLR. (F) Relative filipin fluorescent intensity in cells treated with control siRNA, or siRNA against TDP-43 with control rescue, or cDNA encoding SREBF2 or LDLR. One-way ANOVA with Tukey’s multiple comparisons tests was used to evaluate statistical significance; *, P < 0.05. Quantification was done from at least three independent experiments with 90 cells quantified per experiment. (G) Relative cholesterol level as measured by a fluorometric-based assay in cells treated with control siRNA, or siRNA against TDP-43 with control rescue, or cDNA encoding SREBF2 or LDLR. Three independent experiments were performed, and three biological samples were performed per experiment. One-way ANOVA with Tukey’s multiple comparisons tests was used to evaluate statistical significance; *, P < 0.05; **, P < 0.01.

SREBF2 and LDLR rescue cholesterol reduction caused by TDP-43 deletion. (A) MO3.13 cells were transfected with control siRNA and siRNA against TDP-43 or SREBF2. Cells were stained with TDP-43 (red), SREBF2 (green), and filipin (gray). Scale bar: 10 µm. (B) Relative filipin fluorescent intensity in cells treated with control siRNA and siRNA against TDP-43 or SREBF2. Quantification was done from three independent experiments with 90 cells quantified per experiment. One-way ANOVA with Tukey’s multiple comparisons tests was used to evaluate statistical significance; *, P < 0.05. (C) Total cholesterol level as measured by a fluorometric-based assay in cells treated with control siRNA and siRNA against TDP-43 or SREBF2. n = 3, one-way ANOVA with Tukey’s multiple comparisons tests was used to evaluate statistical significance; *, P < 0.05; **, P < 0.01. (D) Schematic of cDNA rescue experiment under TDP-43 knockdown conditions. (E) Immunoblotting for SREBF2 and LDLR in the rescue experiments. Blue arrow: full-length SREBF2; red arrow: transfected N-terminal domain of SREBF2; orange arrow: N-terminal domain of SREBF2; purple arrow: GFP-tagged LDLR; black arrow: endogenous LDLR. (F) Relative filipin fluorescent intensity in cells treated with control siRNA, or siRNA against TDP-43 with control rescue, or cDNA encoding SREBF2 or LDLR. One-way ANOVA with Tukey’s multiple comparisons tests was used to evaluate statistical significance; *, P < 0.05. Quantification was done from at least three independent experiments with 90 cells quantified per experiment. (G) Relative cholesterol level as measured by a fluorometric-based assay in cells treated with control siRNA, or siRNA against TDP-43 with control rescue, or cDNA encoding SREBF2 or LDLR. Three independent experiments were performed, and three biological samples were performed per experiment. One-way ANOVA with Tukey’s multiple comparisons tests was used to evaluate statistical significance; *, P < 0.05; **, P < 0.01.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal