Reduced SREBF2 and its downstream targets in the TDP-43-deleted oligodendrocytes. (A) Confocal images of RNA-FISH of SREBF2, TDP-43, and LDLR assays using Tardbp^fl/fl (ctrl) and Cnp-Cre;Tardbp^fl/fl (cKO) conditions. Images shown are from dorsal gray, ventral gray, and white matter at P60. Scale bar: 20 µm. (B–D) Quantification of FISH signals across dorsal gray, ventral gray, and white matter for LDLR (B), SREBF2 (C), and TDP-43 (D). Puncta counts of individual oligodendrocytes (faded circle or triangle or square) and means (solid circle or triangle or square) for animal (n = 3) plotted, total mean ± SEM derived from all animals. Significance was tested using unpaired t test; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. n = 3 per genotype, at least three slices per animals were quantified. (E) Confocal images of RNA-FISH of TDP43 and IF of GSTP1 assays using Tardbp^fl/fl (ctrl) and Cnp-Cre;Tardbp^fl/fl (cKO) conditions. Images shown are from the white matter. Scale bar: 20 µm. (F) Quantification of FISH signals in white matter for TDP43 in GSTP1⁺ cells. Puncta counts of individual oligodendrocytes (faded circle or triangle) and means (solid circle or triangle) for each set of experiment (n = 3) plotted, total mean ± SEM derived from all animals. Significance was tested using unpaired t test; ***, P < 0.001. n = 3 per genotype, at least three slices per animals were quantified. cKO, conditional knockout; ctrl, control.