Distinct expression profiles in the 21-d-old spinal cord tissues of Cnp-Cre;Tardbp^fl/fl mice. (A–C) MA plots of pairwise contrasts between Tardbp^fl/fl (ctrl), Cnp-Cre;Tardbp^fl/+ (cHet), and Cnp-Cre;Tardbp^fl/fl (cKO) transcriptomes (P60) revealed patterns of differential expression (FDR-corrected P value <0.1; red: up-regulated; blue: down-regulated) between Cnp-Cre;Tardbp^fl/fl and Tardbp^fl/fl/Cnp-Cre;Tardbp^fl/+ conditions, but not between Tardbp^fl/fl and Cnp-Cre;Tardbp^fl/+ mice. (D–F) MA plots of pairwise contrasts between Tardbp^fl/fl (ctrl), Cnp-Cre;Tardbp^fl/+ (cHet), and Cnp-Cre;Tardbp^fl/fl (cKO) transcriptomes (P21) reveal patterns of differential expression (FDR-corrected P value <0.1; red: up-regulated; blue: down-regulated) between Cnp-Cre;Tardbp^fl/fl and Tardbp^fl/fl/Cnp-Cre;Tardbp^fl/+ conditions, but not between Tardbp^fl/fl and Cnp-Cre;Tardbp^fl/+ mice. (G) PCA of count data across all three conditions shows a clear separation of Cnp-Cre;Tardbp^fl/fl and Tardbp^fl/fl/Cnp-Cre;Tardbp^fl/+ data across the first two principal components at P21. (H) Hierarchical clustering of gene-centered count data cleanly categorizes the data, with Cnp-Cre;Tardbp^fl/fl samples clustered separately from both Tardbp^fl/fl and Cnp-Cre;Tardbp^fl/+ samples at P21. (I) Normalized expression information for myelin-related and knockout-related genes at P21. Error bars describe expression SD. cHet, conditional heterozygous knockout; cKO, conditional knockout; ctrl, control.