Figure 7.
bro1M8 perturbs ILV formation in vivo. (A) Three-dimensional models reconstructed from 200-nm thick-section electron tomograms of bro1Δ (GOY65) with BRO1 or bro1M8 plasmids. The limiting membrane of the MVB is labeled yellow, the ILVs are highlighted in red, and the budding intermediates are colored in green. Scale bars = 100 nm. (B) Quantification of electron tomograms of GOY65 with either BRO1 or bro1M8 plotting ILV size, BP size, and BPs per MVB; 8 MVBs from bro1Δ (GOY65) with BRO1 plasmid containing 6 BPs and 141 ILVs and 10 MVBs from bro1Δ with bro1M8 plasmids containing 23 BPs and 204 ILVs were quantified. Data are represented as mean ± SEM. Asterisks indicate statistically significant differences compared with WT (P < 0.006).

bro1M8 perturbs ILV formation in vivo. (A) Three-dimensional models reconstructed from 200-nm thick-section electron tomograms of bro1Δ (GOY65) with BRO1 or bro1M8 plasmids. The limiting membrane of the MVB is labeled yellow, the ILVs are highlighted in red, and the budding intermediates are colored in green. Scale bars = 100 nm. (B) Quantification of electron tomograms of GOY65 with either BRO1 or bro1M8 plotting ILV size, BP size, and BPs per MVB; 8 MVBs from bro1Δ (GOY65) with BRO1 plasmid containing 6 BPs and 141 ILVs and 10 MVBs from bro1Δ with bro1M8 plasmids containing 23 BPs and 204 ILVs were quantified. Data are represented as mean ± SEM. Asterisks indicate statistically significant differences compared with WT (P < 0.006).

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