Figure 8.
Loss of the α integrin subunits αPS1 or αPS3 gives distinct MET phenotypes. Stage 15 wild-type (a and b), wb (d and e), LanA (g and h), mew (mutant for αPS1, j and k), and scab (mutant for αPS1, m and n) stained for αPS1 and αPS3 (a, b, d, e, g, and h); or Baz, βPS (j, k, m, and n) and E-Cad (k2 and n2). (c, f, i, l, and o) Plots of the average fluorescence intensity (represented as mean gray value) of αPS1 and αPS3 (c, f, and i) or Baz, E-Cad, and βPS (l and o) in either wild-type midgut cells (c) or midgut cells mutant for wb (f), LanA (i), mew (l), or scab (o). Plots are measured along the apical (A) to basal (B) axis of a cell (represented in b, e, h, k, and n by red line). Each n represents the average of 10 cells measured in one embryo (black dotted lines). n = 6 per condition; the mean for each condition is plotted in red. (a–c) αPS1 and αPS3 show distinct localizations in midgut cells, with αPS1 (b2 and c) localizing basally and αPS3 (b1 and c) apically (the small basal peak of αPS3 is in the mesoderm). (d–f) In wb mutants, the polarized localization of both αPS1 (e2 and f) and αPS3 (e1 and f) is lost. (g–i) In LanA mutants, αPS3 levels (h1) are lowered at the apical domain (i), and it shows a more diffuse staining in the cell. While αPS1 localizes predominantly to the basal domain (h2), its levels are just over half that in wild type (i). (j–l) In mutants for αPS1, mew, the midgut looks very similar to in wb mutants: cells fail to form an epithelial monolayer, and Baz (k1), E-Cad (k2), and βPS (k3) are found delocalized throughout the cells. (l) Accordingly, the apical peaks of Baz and E-Cad are lost in mew mutants. There is just a small peak of βPS apically and a low broad peak basally which correlates with diffuse βPS throughout the mesoderm layer. (m–o) In scab mutants, the midgut looks very similar to that in LanA mutants: midgut cells form a disorganized-looking monolayer of columnar or wedge-shaped cells. (n and o) Baz and E-Cad levels reduced, with their apical peaks half the height of those in wild type. βPS integrin staining is completely lost from the apical side of the cell, but only slightly reduced on the basal side (n3 and o). White dashed lines in a, d, g, j, and m indicate the apical side of the midgut, and yellow lines, the basal. Red boxes in a, d, g, j, and m depict the area of the midgut epithelium shown in b, e, h, k, and n. Confocal images are oriented with the anterior to the left and posterior to the right. Scale bars, 10 µm.

Loss of the α integrin subunits αPS1 or αPS3 gives distinct MET phenotypes. Stage 15 wild-type (a and b), wb (d and e), LanA (g and h), mew (mutant for αPS1, j and k), and scab (mutant for αPS1, m and n) stained for αPS1 and αPS3 (a, b, d, e, g, and h); or Baz, βPS (j, k, m, and n) and E-Cad (k2 and n2). (c, f, i, l, and o) Plots of the average fluorescence intensity (represented as mean gray value) of αPS1 and αPS3 (c, f, and i) or Baz, E-Cad, and βPS (l and o) in either wild-type midgut cells (c) or midgut cells mutant for wb (f), LanA (i), mew (l), or scab (o). Plots are measured along the apical (A) to basal (B) axis of a cell (represented in b, e, h, k, and n by red line). Each n represents the average of 10 cells measured in one embryo (black dotted lines). n = 6 per condition; the mean for each condition is plotted in red. (a–c) αPS1 and αPS3 show distinct localizations in midgut cells, with αPS1 (b2 and c) localizing basally and αPS3 (b1 and c) apically (the small basal peak of αPS3 is in the mesoderm). (d–f) In wb mutants, the polarized localization of both αPS1 (e2 and f) and αPS3 (e1 and f) is lost. (g–i) In LanA mutants, αPS3 levels (h1) are lowered at the apical domain (i), and it shows a more diffuse staining in the cell. While αPS1 localizes predominantly to the basal domain (h2), its levels are just over half that in wild type (i). (j–l) In mutants for αPS1, mew, the midgut looks very similar to in wb mutants: cells fail to form an epithelial monolayer, and Baz (k1), E-Cad (k2), and βPS (k3) are found delocalized throughout the cells. (l) Accordingly, the apical peaks of Baz and E-Cad are lost in mew mutants. There is just a small peak of βPS apically and a low broad peak basally which correlates with diffuse βPS throughout the mesoderm layer. (m–o) In scab mutants, the midgut looks very similar to that in LanA mutants: midgut cells form a disorganized-looking monolayer of columnar or wedge-shaped cells. (n and o) Baz and E-Cad levels reduced, with their apical peaks half the height of those in wild type. βPS integrin staining is completely lost from the apical side of the cell, but only slightly reduced on the basal side (n3 and o). White dashed lines in a, d, g, j, and m indicate the apical side of the midgut, and yellow lines, the basal. Red boxes in a, d, g, j, and m depict the area of the midgut epithelium shown in b, e, h, k, and n. Confocal images are oriented with the anterior to the left and posterior to the right. Scale bars, 10 µm.

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