FWHM and amplitude (maximum height) of fluorescent intensity peaks. (a–d) Measurements of the FWHM determine the spread away from the peak, and the amplitude of fluorescent intensity peaks indicates relative protein concentrations. Data are displayed as scatter plots: each dot represents the average of a minimum of 10 cells measured in one embryo, with mean and SD from the mean indicated by horizontal lines. Each n represents the average of 10 cells measured in one embryo. n = 6 per condition. Statistical analyses were performed comparing mutants to the wild-type (Wt) for each peak using unpaired two-tailed t test. ***, P ≤ 0.001; **, P ≤ 0.01; *, P ≤ 0.05; no asterisks, nonsignificant.