Figure 8.
The region downstream of the CM1 domain in human CDK5RAP2 is inhibitory for binding to γ-TuRCs. (A) Cartoon depicting the various CDK5RAP2 N-terminal fragments used in IP experiments and indicating their relative γ-TuRC binding affinity. (B and C) Western blots of co-IP experiments from HEK cell extracts probed for the various GFP-tagged CDK5RAP2 fragments (top) and γ-tubulin (bottom).

The region downstream of the CM1 domain in human CDK5RAP2 is inhibitory for binding to γ-TuRCs. (A) Cartoon depicting the various CDK5RAP2 N-terminal fragments used in IP experiments and indicating their relative γ-TuRC binding affinity. (B and C) Western blots of co-IP experiments from HEK cell extracts probed for the various GFP-tagged CDK5RAP2 fragments (top) and γ-tubulin (bottom).

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