Expression of pUbq-Cnn-C^T increases the frequency of nuclear and spindle defects observed within syncytial embryos. (A–F) Fluorescence images of syncytial embryos expressing either pUbq-Cnn-C (A–C) or pUbq-Cnn-C^T (D–F) in either S-phase/prophase (A and D) Metaphase (B and E), or telophase (C and F). Note the apparent high density of cytosolic microtubules that can be (but are not always) observed in pUbq-Cnn-C^T embryos, along with major organization defects. (G) Graph showing results from a blind categorization of WT (n = 49), pUbq-Cnn-C (n = 88), or pUbq-Cnn-C^T (n = 36) embryos based on the presence or absence of moderate or severe nuclear or spindle defects. (H) Fluorescence images of an egg expressing the microtubule marker Jupiter-mCherry (magenta) that had been injected with mRNA encoding GFP-Cnn-T-N (green).