Polarity is established normally in pUbq-Cnn-C^T oocytes. (A and B) Fluorescence images show localization of Staufen protein in oocytes expressing pUbq-Cnn-C (A) or pUbq-Cnn-C^T (B) at stages 8, 9, and 10, as indicated. Staufen localized in the center of the oocyte at stage 8 and then at the posterior in stage 9 and 10 in all pUbq-Cnn-C (n = 35, stage 8; n = 35, stage 9; n = 30, stage 10) and all pUbq-Cnn-C^T (n = 40, stage 8; n = 50, stage 9; n = 40, stage 10) oocytes that were imaged. (C) Fluorescence images show localization of Gurken protein in oocytes expressing pUbq-Cnn-C or pUbq-Cnn-C^T at stage 9. Gurken protein was positioned close to the nucleus in the dorsal corner in all pUbq-Cnn-C (n = 30) and all pUbq-Cnn-C^T (n = 35) stage 9 oocytes. Gurken mispositioning or its absence results in abnormal dorsal appendages that protrude from the surface of the egg, but the dorsal appendages were normal on all pUbq-Cnn-C (n = 724) and all pUbq-Cnn-C^T (n = 488) eggs.