The γ-TuRC–specific protein Grip75^GCP4 is recruited strongly to Cnn-T and Cnn-C^Δ1-77 scaffolds. (A–C) Fluorescence images show mKATE-Cnn scaffolds of different types, as indicated, within eggs expressing endogenously tagged Grip75^GCP4-sfGFP. Insets show representative examples of individual scaffolds. (D) Graph showing fluorescence intensity measurements (in arbitrary units) of Grip75^GCP4-sfGFP and mKATE-Cnn at Cnn-C (n = 1,920 scaffolds; 12 eggs), Cnn-T (n = 1,650 scaffolds; 10 eggs), or Cnn-C^Δ1-77 (n = 2,599 scaffolds; 10 eggs). Each dot represents a single scaffold. (E) Graph shows slope values of linear regression lines calculated for scaffolds of different types. Each slope value represents an individual egg that contained multiple scaffolds. The mean and 95% CIs are indicated. P values are from comparisons to the Cnn-C mean using a one-way ANOVA.