Diagrams of different Cnn constructs (omitting the tags) used in this study. (A) Diagram showing full-length Cnn constructs without modifications to the PReM domain. Cnn-T is the testes-specific isoform in Drosophila. Cnn-C is the major centrosomal isoform in Drosophila. Cnn-C^T represents an artificial form of Cnn-C in which the N-terminal region of Cnn-C (dark blue) has been replaced with the N-terminal region of Cnn-T (red). (B) Diagram showing Cnn constructs used in the scaffold assay, where Cnn-C contains phosphmimetic mutations in the PReM domain to drive scaffold formation in vivo. (C) Diagram showing bacterially purified N-terminal fragments of different Cnn types used in co-IP experiments.