![Epithelial NMIIA deficiency leads to persistence of the contractile epidermal ring. (A) Optical sections (planar views) of confocal images of representative Myh9 cKO and control littermate embryos labeled with EpCAM (green) at hillock (E12.5) and bulb (E13.5) stages. Scale bar, 50 µm. (B) Heatmap of cell roundness in the planar images shown in A. (C and D) Quantification of cell roundness (C) and aspect ratio (D) of cells adjacent to the bud (0–30 µm ∼ ring cells) and those farther away from the bud (70–100 µm, control epidermal cells [EC]) in 6 or 7 control and 7–11 Myh9 cKO embryos per stage (Control: nE12.5 Ring cells = 931, nE12.5 EC = 448, nE13.5 Ring cells = 697, nE13.5 EC= 921; Myh9 cKO: nE12.5 Ring cells = 886, nE12.5 EC = 219, nE13.5 Ring cells = 1,174, nE13.5 EC = 1,201). Data shown represent median (line) with 25th and 75th percentiles (hinges) plus 1.5× interquartile ranges (whiskers). Statistical significance was assessed with the Mann-Whitney U test with Bonferroni correction. **, P ≤ 0.01; ****, P ≤ 0.0001.](https://cdn.rupress.org/rup/content_public/journal/jcb/220/8/10.1083_jcb.202008062/4/jcb_202008062_fig7.png?Expires=1771577316&Signature=GQ85FII7c4SLekTcmwWs6JKYt0J4K3Jx~nQoyqSblxTZkE06rryfICxS5gFCDw~-nhe9TNruiqDs3ygkS0Gd7~DDCh2NR13Uy9I8GK26zkLiKv1MogmPfzakX5UcXp2PoAGassDQZgctqjG3XGwb-3ejO4mtEiht0ASk-rx6U1~RgL4V4lzuEq3WctQ0w0HXSf2ufB~l5pk7ibBWtlbNBxlVrT-a2KUFiYynGRWCD7MM2FFnTiMlZf7BpvGq9S~bKtczOEvFV3hg310mIKn77RNUvOtUfmCsLq4L22s4O4mdg9yFJfAz9V~kPHxpMjzS1sDzcZXnI5hOCHh2czRF9w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Epithelial NMIIA deficiency leads to persistence of the contractile epidermal ring. (A) Optical sections (planar views) of confocal images of representative Myh9 cKO and control littermate embryos labeled with EpCAM (green) at hillock (E12.5) and bulb (E13.5) stages. Scale bar, 50 µm. (B) Heatmap of cell roundness in the planar images shown in A. (C and D) Quantification of cell roundness (C) and aspect ratio (D) of cells adjacent to the bud (0–30 µm ∼ ring cells) and those farther away from the bud (70–100 µm, control epidermal cells [EC]) in 6 or 7 control and 7–11 Myh9 cKO embryos per stage (Control: nE12.5 Ring cells = 931, nE12.5 EC = 448, nE13.5 Ring cells = 697, nE13.5 EC= 921; Myh9 cKO: nE12.5 Ring cells = 886, nE12.5 EC = 219, nE13.5 Ring cells = 1,174, nE13.5 EC = 1,201). Data shown represent median (line) with 25th and 75th percentiles (hinges) plus 1.5× interquartile ranges (whiskers). Statistical significance was assessed with the Mann-Whitney U test with Bonferroni correction. **, P ≤ 0.01; ****, P ≤ 0.0001.
