Expression of mammary fate markers at placode stage. (A) Planar and sagittal views of confocal whole-mount images of samples from TOP-gal, NF-κB-gal, FGF20β-gal/wt, and TCF/Lef1:H2B-GFP reporter mouse models or wild-type mouse at E11.5 (arrowhead indicates section plane). Expression of β-galactosidase or Lef1 was detected by the immunofluorescent staining (green) in the β-gal reporter or wild-type samples, respectively. All samples were stained with epithelial marker EpCAM (white) and nuclear marker Hoechst (cyan). Dashed line marks the epithelial-mesenchymal border. Scale bar, 50 µm. (B) Planar view showing maximum intensity projections of epithelial tissue (K14-Cre; mTmG) at E11.5. Surface rendering (white) of mammary placode 3 in respect to body axes. Scale bar, 20 µm.
