Figure 1.
Growth of the mammary rudiment during early developmental stages. (A) Optical sections (planar and sagittal views; arrowhead indicates section plane) of confocal microscopy images from K14-Cre/mTmG mouse mammary rudiments at placode (E11.5), hillock (E12.5), and bulb (E13.5) stages; epithelium in green (membrane-bound GFP and EpCAM) and mesenchyme in red. Dashed line marks the epithelial-mesenchymal border. Scale bar, 50 µm. (B and C) Planar and sagittal views of maximum intensity projections of epithelial tissue; rendered surfaces of mammary rudiments in white. Scale bar, 20 µm. (D and E) Quantification of volume (D) and sphericity (E) of mammary rudiments using rendered surfaces. One-way ANOVA with Šidák’s post hoc comparison; ***, P ≤ 0.001; ****, P ≤ 0.0001; nE11.5 = 8; nE12.5 = 8; nE13.5 = 10. Data are presented as mean ± SD.

Growth of the mammary rudiment during early developmental stages. (A) Optical sections (planar and sagittal views; arrowhead indicates section plane) of confocal microscopy images from K14-Cre/mTmG mouse mammary rudiments at placode (E11.5), hillock (E12.5), and bulb (E13.5) stages; epithelium in green (membrane-bound GFP and EpCAM) and mesenchyme in red. Dashed line marks the epithelial-mesenchymal border. Scale bar, 50 µm. (B and C) Planar and sagittal views of maximum intensity projections of epithelial tissue; rendered surfaces of mammary rudiments in white. Scale bar, 20 µm. (D and E) Quantification of volume (D) and sphericity (E) of mammary rudiments using rendered surfaces. One-way ANOVA with Šidák’s post hoc comparison; ***, P ≤ 0.001; ****, P ≤ 0.0001; nE11.5 = 8; nE12.5 = 8; nE13.5 = 10. Data are presented as mean ± SD.

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