WDR62 interacts with katanin at spindle poles. (A) Mass spectrometry results of streptavidin pull down from HEK293T cells transiently transfected with WDR62-GFP-Bio and synchronized in mitosis by nocodazole treatment. (B) Immunofluorescence staining of p80 and DNA (DAPI) in WDR62-GFP-Strep knock-in HeLa cells during mitosis. (C–E) Immunofluorescence staining and quantification of WDR62 and p80 intensities at spindle poles in control or indicated knockout (KO) HeLa cells. For WDR62 intensity, control, n = 31 spindle poles; p80 knockout, n = 44. For p80 intensity, control, n = 34; WDR62 knockout, n = 47. Scale bars, 2 µm. Data represent mean ± SD. ***, P < 0.001; two-tailed t test.