Figure 5.
WDR62 loss leaves k-fiber plus-end dynamics intact but leads to wider metaphase plates.(A–H) Automated sister-kinetochore tracking of RPE1 GFP-centrin1/GFP-CENPA cells treated with indicated siRNA. Shown are kymographs single, oscillating sister-kinetochores, scale bars = 1 µm (A); quantification of the interkinetochore distances (B); schematic illustrating the parameters of sister-kinetochore oscillation autocorrelation curves (C); autocorrelation curves of sister-kinetochore oscillations along spindle axis (D); sister-kinetochore velocities (E); mean metaphase plate width (F); maximal metaphase plate width over time (G); and mean metaphase plate width at anaphase onset (H). N = 3–5, n = 35 (siCTRL), 12 (siCAPD2), 34 (siKATNB1), and 33 (siWDR62) cells; dot plots represent each cell with bars displaying mean ± SEM; box plot represent mean ± SD; *, P < 0.05; ****, P < 0.0001, one-way ANOVA. AU, arbitrary units.

WDR62 loss leaves k-fiber plus-end dynamics intact but leads to wider metaphase plates. (A–H) Automated sister-kinetochore tracking of RPE1 GFP-centrin1/GFP-CENPA cells treated with indicated siRNA. Shown are kymographs single, oscillating sister-kinetochores, scale bars = 1 µm (A); quantification of the interkinetochore distances (B); schematic illustrating the parameters of sister-kinetochore oscillation autocorrelation curves (C); autocorrelation curves of sister-kinetochore oscillations along spindle axis (D); sister-kinetochore velocities (E); mean metaphase plate width (F); maximal metaphase plate width over time (G); and mean metaphase plate width at anaphase onset (H). N = 3–5, n = 35 (siCTRL), 12 (siCAPD2), 34 (siKATNB1), and 33 (siWDR62) cells; dot plots represent each cell with bars displaying mean ± SEM; box plot represent mean ± SD; *, P < 0.05; ****, P < 0.0001, one-way ANOVA. AU, arbitrary units.

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