WDR62 deletion and depletion efficiency. Related to Fig. 2. (A) Genomic sequence before and after performing CRISPR-Cas9 WDR62 knockout on HeLa H2B-GFP cells. PAM indicates the protospacer adjacent motif GGG. (B) Immunofluorescence images of metaphase WDR62^+/+ and WDR62^−/− HeLa H2B-GFP cells, stained with WDR62 and pericentrin antibodies and DAPI (left); quantification of WDR62 levels relative to WDR62^+/+ (right): N = 5, n = 310 (WDR62^+/+) and 322 (WDR62^−/−) cells; dot plot represents median per experiment; bars represent mean ± SEM; ****, P < 0.0001, two-tailed unpaired t test. (C) Immunofluorescence images of metaphase RPE1 cells treated with siCTRL and siWDR62, stained with WDR62 and pericentrin antibodies and DAPI (left); quantification of WDR62 levels after siWDR62 treatment relative to siCTRL (right): N = 5, n = 310 cells per conditions; dot plot represents median per experiment; bars represent mean ± SEM; ****, P < 0.0001, two-tailed paired t test. All scale bars = 5 µm.