Figure S3.
A Golgi-related compartment localizes to microtubule minus ends in basal process varicosities. (A) Localization of GalNacT2-mCherry, GFP-Rab6A, and ManII-GFP in aRG apical process. N, nucleus. (B) Localization of GalNacT2-mCherry, GalT-mCherry, and GFP-Rab6A, TGN46-GFP, GMAP210-GFP, and ManII-GFP in aRG basal process varicosities. (C) Quantification of the percentage of basal process varicosities positive for GalNacT2 (n = 70 varicosities in 13 cells). (D) Immunostaining for GalNacT2 in Vimentin-stained cortical tissue. (E) Immunostaining for GalNacT2, together with Sox2 and α-tubulin, in human RG cells cultured in vitro. (F) Co-expression of GalNacT2-mCherry and GalT-mCherry with CAMSAP3-GFP in basal process of mouse aRG cells. (G) Live imaging of EB3-GFP and GalNacT2-mCherry in mouse aRG cell basal process and corresponding kymograph. (H) Quantification of the percentage of EB3 comets emanating from GalNacT2-positive foci throughout the basal process, in shafts and in varicosities (n = 333 comets from 28 cells). (I) Directionality of microtubule growth depending on whether EB3 comet emanates within or away from GalNacT2-positive foci (n = 274 comets from 28 cells). ****, P < 0.0001 by Mann-Whitney tests. All experiments were performed in at least three independent mice or two independent human samples. Error bars indicate SD.

A Golgi-related compartment localizes to microtubule minus ends in basal process varicosities. (A) Localization of GalNacT2-mCherry, GFP-Rab6A, and ManII-GFP in aRG apical process. N, nucleus. (B) Localization of GalNacT2-mCherry, GalT-mCherry, and GFP-Rab6A, TGN46-GFP, GMAP210-GFP, and ManII-GFP in aRG basal process varicosities. (C) Quantification of the percentage of basal process varicosities positive for GalNacT2 (n = 70 varicosities in 13 cells). (D) Immunostaining for GalNacT2 in Vimentin-stained cortical tissue. (E) Immunostaining for GalNacT2, together with Sox2 and α-tubulin, in human RG cells cultured in vitro. (F) Co-expression of GalNacT2-mCherry and GalT-mCherry with CAMSAP3-GFP in basal process of mouse aRG cells. (G) Live imaging of EB3-GFP and GalNacT2-mCherry in mouse aRG cell basal process and corresponding kymograph. (H) Quantification of the percentage of EB3 comets emanating from GalNacT2-positive foci throughout the basal process, in shafts and in varicosities (n = 333 comets from 28 cells). (I) Directionality of microtubule growth depending on whether EB3 comet emanates within or away from GalNacT2-positive foci (n = 274 comets from 28 cells). ****, P < 0.0001 by Mann-Whitney tests. All experiments were performed in at least three independent mice or two independent human samples. Error bars indicate SD.

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