CAMSAP1 and 2 KD alters microtubule growth from varicosities and basal process architecture. (A) KD of CAMSAP1 and 2 destabilizes the basal process. E14.5 embryos were in utero electroporated with shRNA-expressing plasmids or scramble control and fixed at E15.5 and E16.5. Brains were stained for DAPI or NeuN to visualize basal surface. Dashed lines indicate apical (bottom) and basal (top) surfaces. (B) Live imaging of EB3-GFP in varicosities of RG cells expressing CAMSAP1 shRNA, CAMSAP2 shRNA, or scramble control. Right: Kymographs highlighting reduced EB3 comet formation in CAMSAP1 and 2 KD. Arrowheads: newly formed EB3 comets. (C) Quantification of the rate of EB3 comet formation within varicosities of scramble shRNA (n = 42 varicosities), CAMSAP1 shRNA (n = 35 varicosities), and CAMSAP2 shRNA (n = 38 varicosities). (D) Quantification of the rate of EB3 comet formation within shafts of scramble shRNA (n = 41 varicosities), CAMSAP1 shRNA (n = 35 varicosities), and CAMSAP2 shRNA (n = 38 varicosities). ***, P < 0.001; ****, P < 0.0001 by Mann-Whitney tests. All experiments were performed in nine brains from three independent mice.