CAMSAP 1, 2, and 3 endogenous localization and KD validation. Related to Fig. 4 and Fig. 5. (A) Immunostaining for CAMSAP1, CAMSAP2, and CAMSAP3 in GFP-electroporated mouse cortical tissue. (B) Immunostaining for CAMSAP1 and CAMSAP2 in GFP-electroporated human cortical tissue. (C) Immunostaining for CAMSAP1 and CAMSAP3, together with Sox2 and α-tubulin, in human RG cells cultured in vitro. (D) Directionality of microtubule growth depending on whether EB3 comet emanates within or away from CAMSAP3-positive foci (n = 252 comets from 15 cells). (E) Quantitative PCR measurement of CAMSAP 1 and 2 mRNA levels following KD in Neuro2A cells. ****, P <0.0001. Error bars indicate SD. A.U., arbitrary units.