Nondeveloping embryos with abnormalities characteristic of egg chambers that do not eliminate NCN. (A) Graph indicating percentage of stage 11–13 and stage 14 egg chambers with abnormal morphology laid by 3- and 10-d-old females, and percentage of unhatched embryos laid by females of mixed age, 3 and 10 d after eclosion with normal or abnormal morphology. (B) Violin plot showing distributions of distances between dorsal appendages of inviable embryos that either developed to organogenesis stages (normal) or appeared to be unfertilized (abnormal). (C and D) Embryos with normal (C) and abnormal (D) morphology, with distance between developing dorsal appendages marked and autofluorescent internal organs indicated by arrow. Scale bar: 100 µm (bar in C for C and D).