Abnormalities of egg chambers that do not eliminate NCN. (A–D) Stage 12 (A and B) and stage 13 (C and D )egg chambers marked with nuclear GFP fluorescence (green) and stained with DAPI (white) with 13 (A and C) and 15 (B and D) NCN, respectively, with distance between developing dorsal appendages marked. (E) Violin plot showing distributions of distances between dorsal appendages of stage 12/13 oocytes with either 13 or 15 NCN (NC nuc). (F and G) Stage 14 egg chambers with normal (F) and abnormal morphology (G). (H–K) Egg chambers (MTD-Gal4 UASp-NLSGFP, DAPI) identified at stage 13 as morphologically abnormal (H) or normal (J) and then fixed for imaging, or cultured for 5+ h at RT after morphological characterization as abnormal (I) or normal (K). Scale bar: 50 µm (bar in A for A–D and F–K).