Anatomy of the nuclear membrane of the entering NC nucleus and fine structure of anterior face of a stage 11 oocyte. (A–A″′) The entering NC nucleus of the stage 10B oocyte is imaged with DAPI (white, A), nuclear GFP (green, A′), phalloidin (blue, A″), and α-nucleoporin, which stains only the portion of the nucleus that has not entered (A″′). (B and B′) Entering NC nucleus stained with α-Msp300 (red) and α-lamin (green) antibodies. (C) EM of entering NC nucleus (position in egg chamber indicated by cartoon) with red-lined region shown at high magnification in C′. (D and D′) Nuclear GFP (green) and DAPI (white) marks NC nucleus in the ooplasm, border cells, and GV. (E–I) Successive sections imaged by electron microscopy of a stage 11 egg chamber showing contiguous plasma membrane at the anterior oocyte face in outer sections (E and I) and channel in intermediate sections (F–H). (F′) Higher-magnification image of red-lined boxed region in F showing NC, follicle cells, vitelline membrane (VM), oocyte, and channel. Scale bars: 50 µm (bar in A for A–B′, bar in D for D and D′); 20 µm (E–I); 10 µm (C); 1 µm (C′); 10 µm (F′).