Enucleated NCs in stage 10B and stage 11 egg chambers. (A and B) Enucleated NCs indicated (arrows) in stage 10B chambers expressing myristoylated-GFP and stained with DAPI (A) or expressing nuclear-GFP and stained with phalloidin (B). (C) Enucleated NC of stage 11 egg chamber stained with phalloidin and DAPI. (D) Graph showing volumes of stage 2–13 egg chambers (80 egg chambers analyzed; data in Table S2). Scale bar: 50 µm for A–C.