Figure 3. NC content in Drosophila egg... | Rockefeller University Press

Figure 3.

$NC content in Drosophila egg chambers.(A–G) Analysis of D. melanogaster stage 10A–12 egg chambers expressing nuclear GFP (green) and stained with DAPI (white); orientation anterior up. (A) Stage 10A oocyte has no ectopic nuclei; numbering indicates lineage as defined in Fig. 2 A. (B–D) Stage 10B with one (B), two (C), or three (D) ectopic oocyte nuclei; NC numbering: 1–15 from most anterior, not according to lineage. (E) Pie chart indicating fraction of 18 stage 11 egg chambers that have 12, 13, 14, or 15 NCN. (F and G) Stage 11 (F) and stage 12 (G) egg chambers with 13 NCN and no ectopic nuclei in the oocyte. NC numbering: 1–15 from most anterior. (H–K)D. simulans, D. hydei, and D. virilis egg chambers stained with DAPI (white) with two ectopic NCN in the stage 10B D. simulans oocyte (H) and 13 NCN in the D. simulans (I), D. hydei (J), and D. virilis (K) stage 11 egg chambers. Scale bar: 50 µm. (L–Q) Midstage 10 (L and M), late stage 10 (N and O), and stage 11 (P and Q) egg chambers (MTD-Gal4 UASp-NLSGFP) viewed with transmitted light (L, N, and P) and fluorescence microscopy (M, O, and Q). Orientation anterior to top or figure and as indicated. Egg chambers were viewed under a dissecting microscope during ex vivo incubation and distinguished both by relative size of the oocyte and by opacity of anterior ooplasm for identification of GV at midstage 10 (L), entering NCN at late stage 10B (N), and stage 11 (P). Egg chambers were photographed at the three indicated stages or were fixed, stained with DAPI, and mounted for imaging with a confocal microscope. Timing was ≤10 min after isolation (L and M), 10–20 min after appearance of GV at anterior oocyte membrane (N and O), and 7–17 min after appearance of broad anterior clearing (P and Q). Scale bars: 50 µm.$

NC content in Drosophila egg chambers. (A–G) Analysis of D. melanogaster stage 10A–12 egg chambers expressing nuclear GFP (green) and stained with DAPI (white); orientation anterior up. (A) Stage 10A oocyte has no ectopic nuclei; numbering indicates lineage as defined in Fig. 2 A. (B–D) Stage 10B with one (B), two (C), or three (D) ectopic oocyte nuclei; NC numbering: 1–15 from most anterior, not according to lineage. (E) Pie chart indicating fraction of 18 stage 11 egg chambers that have 12, 13, 14, or 15 NCN. (F and G) Stage 11 (F) and stage 12 (G) egg chambers with 13 NCN and no ectopic nuclei in the oocyte. NC numbering: 1–15 from most anterior. (H–K)D. simulans, D. hydei, and D. virilis egg chambers stained with DAPI (white) with two ectopic NCN in the stage 10B D. simulans oocyte (H) and 13 NCN in the D. simulans (I), D. hydei (J), and D. virilis (K) stage 11 egg chambers. Scale bar: 50 µm. (L–Q) Midstage 10 (L and M), late stage 10 (N and O), and stage 11 (P and Q) egg chambers (MTD-Gal4 UASp-NLSGFP) viewed with transmitted light (L, N, and P) and fluorescence microscopy (M, O, and Q). Orientation anterior to top or figure and as indicated. Egg chambers were viewed under a dissecting microscope during ex vivo incubation and distinguished both by relative size of the oocyte and by opacity of anterior ooplasm for identification of GV at midstage 10 (L), entering NCN at late stage 10B (N), and stage 11 (P). Egg chambers were photographed at the three indicated stages or were fixed, stained with DAPI, and mounted for imaging with a confocal microscope. Timing was ≤10 min after isolation (L and M), 10–20 min after appearance of GV at anterior oocyte membrane (N and O), and 7–17 min after appearance of broad anterior clearing (P and Q). Scale bars: 50 µm.