Defined positions of NCN. (A) Lineage tree of NCs; oocyte (1; blue), oocyte daughters (2, 3, 5, 9; tier 1, red), oocyte granddaughters (4, 6, 7, 10, 11, 13; tier 2, green), oocyte great-granddaughters (8, 12, 14, 15, 16; tier 3, yellow); tiers 1, 2, and 3 are touching the oocyte, in middle NC region, farthest from oocyte, respectively. (B–E) Orientation anterior up; number cell numbering in all panels relate to lineage indicated in A. (B) Stage 3 egg chamber with nuclei and RCs identities marked. (B′) Cartoon of egg chamber in B. (C–C″) Stage 10A egg chamber with identities of nuclei (C) and RCs marked (C′) and depicted in cartoon (C″). (D and D′) Stage 10B depicted in 3D projection (D) and cartoon (D′). (E) Stage 12 egg chamber depicted in cartoon. Genotype: MTD-Gal4 UASp-nucGFP.