NCN in stage 10 oocytes. (A) Graph tabulating egg chamber stages in WT ovaries of females raised under normal conditions (59 ovary lobes) or conditions optimized for stages late 10B (with NCN in ooplasm) and 11 (54 ovary lobes). (B and B′) Stage 10A egg chambers with germline expression of nuclear GFP (green) in 15 NCN and GV, stained with α-lamin antibody (red) and DAPI (white); orientation anterior up. NC numbering: 1–15 from most anterior. (C) Stage 10B WT egg chamber, fixed and stained with DAPI; arrows indicate GV and NCN in ooplasm; orientation anterior left. (D) Fixed stage 10B egg chamber stained with DAPI (white) with germline expression of nuclear GFP, and one entering NC nucleus (arrow); orientation anterior up. (E) Unfixed (live) stage 10B egg chamber with germline expression of nuclear GFP (green) in 15 NCN, one in ooplasm (arrow); orientation anterior up. Scale bars: 50 µm.